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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
29/09/2014 |
Actualizado : |
24/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
ISSN : |
0717-3458 |
DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
Thesagro : |
CITRUS; CYDIA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02405naa a2200241 a 4500 001 1050705 005 2021-06-24 008 2012 bl uuuu u00u1 u #d 022 $a0717-3458 024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI 100 1 $aFEDERICI, M. 245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource] 260 $c2012 520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. 650 $aCITRUS 650 $aCYDIA 700 1 $aMARCONDES, J.A. 700 1 $aPICCHI, S.C. 700 1 $aSTUCHI, E.S. 700 1 $aFADEL, A.L. 700 1 $aLAIA, M.L. 700 1 $aLEMOS, M.V.F. 773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
28/12/2018 |
Actualizado : |
02/01/2019 |
Tipo de producción científica : |
Poster |
Autor : |
BRANDARIZ, S.P.; GONZÁLEZ-REYMÚNDEZ, A.; LADO, B.; QUINCKE, M.; VON ZITZEWITZ, J.; CASTRO, M.; MATUS, I.; DEL POZO, A.; GUTIÉRREZ , L. |
Afiliación : |
Departamento de Estadística, Biometría y Cómputos. Facultad de Agronomía, Universidad de la República. Garzón 780, Montevideo 12900, Uruguay.; Departamento de Estadística, Biometría y Cómputos. Facultad de Agronomía, Universidad de la República. Garzón 780, Montevideo 12900, Uruguay,; BETTINA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Departamento de Estadística, Biometría y Cómputos. Facultad de Agronomía, Universidad de la República. Garzón 780, Montevideo 12900, Uruguay,; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JARISLAV RAMON VON ZITZEWITZ VON SALVIATI, Secobra Saatzucht GmbH Feldkirchen 3, 85368 Moosburg, Germany,; MARINA CASTRO DERENYI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Investigaciones Agropecuarias, Centro Regional de Investigación Quilamapu, Casilla 426, Chillán, Chile.; Facultad de Ciencias Agrarias, Universidad de Talca, Casilla 747, Talca, Chile.; LUCCÍA GUTIÉRREZ, Departamento de Estadística, Biometría y Cómputos. Facultad de Agronomía, Universidad de la República. Garzón 780, Montevideo 12900, Uruguay. |
Título : |
Effect of using imputed missing data on QTL detection on a wheat GWAS panel. [Poster]. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
In: German, S.; Quincke, M.; Vázquez, D.; Castro, M.; Pereyra, S.; Silva, P.; García, A. (Eds.). Seminario Internacional "1914-2014: Un siglo de mejoramiento de trigo en La Estanzuela". Montevideo (UY): INIA, 2018. |
Páginas : |
p. 304. |
Serie : |
(INIA Serie Técnica; 241). |
ISSN : |
1688-9266 |
Idioma : |
Español |
Thesagro : |
TRIGO. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12228/1/st-241-2018.p.304-Brandariz-et-al.pdf
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Marc : |
LEADER 00870nam a2200241 a 4500 001 1059393 005 2019-01-02 008 2018 bl uuuu u00u1 u #d 022 $a1688-9266 100 1 $aBRANDARIZ, S.P. 245 $aEffect of using imputed missing data on QTL detection on a wheat GWAS panel. [Poster].$h[electronic resource] 260 $aIn: German, S.; Quincke, M.; Vázquez, D.; Castro, M.; Pereyra, S.; Silva, P.; García, A. (Eds.). Seminario Internacional "1914-2014: Un siglo de mejoramiento de trigo en La Estanzuela". Montevideo (UY): INIA$c2018 300 $ap. 304. 490 $a(INIA Serie Técnica; 241). 650 $aTRIGO 700 1 $aGONZÁLEZ-REYMÚNDEZ, A. 700 1 $aLADO, B. 700 1 $aQUINCKE, M. 700 1 $aVON ZITZEWITZ, J. 700 1 $aCASTRO, M. 700 1 $aMATUS, I. 700 1 $aDEL POZO, A. 700 1 $aGUTIÉRREZ , L.
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